Location, location, location: Use of CRISPR-Cas9 for genome editing in human pathogenic fungi
نویسندگان
چکیده
منابع مشابه
Location, location, location: Use of CRISPR-Cas9 for genome editing in human pathogenic fungi
Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas9 systems enable the targeting of a double-strand break in genomic DNA to a location chosen by the investigator [1]. The break may be repaired (Fig 1) by cellular nonhomologous end joining (NHEJ) machinery to yield small insertions and deletions [2], which are often loss-of-function mutations in coding regions. If a repair tem...
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ژورنال
عنوان ژورنال: PLOS Pathogens
سال: 2017
ISSN: 1553-7374
DOI: 10.1371/journal.ppat.1006209